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Triticum vulgaris lectin (WGA)
Article No | Product name | Pack Size |
---|---|---|
05-0102-1000 | Absolute Lectins | 1000 mg |
05-0102-10 | WGA | 10 mg |
05-0102-25 | WGA | 25 mg |
05-0102-100 | WGA | 100 mg |
特征
產品描述
Triticum vulgaris lectin or Weat germ agglutinin (WGA) is isolated from Triticum vulgaris (Weat germ) and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 36 kDa. WGA selectively binds to N-Acetyl glucosamine (GlcNAc) and to N-acetylneuraminic acid (sialic acid) residues of glycoproteins and glycolipids (1).
The WGA lectin agglutinates erythrocytes and most types of malignant cells. It agglutinates rabbit erythrocytes at < 0.1 µg/ml following trypsin treatment of the cells. Adding 300 mM N-Acetyle-D-Glucosamine gives an inhibition with a titer that is at least 8-fold lower than the control.
Wheat germ agglutinin inhibits the C receptor interaction, which has implications in studies of receptor micro-heterogeneity and ligand binding sites (1).
WGA together with Con A is the lectin most widely used as an analytical and preparative agent when studying glycoproteins and cell surface proteins. The immobilized lectin can be used for affinity chromatography of cells and sub-cellular particles.
Medicago’s WGA is supplied as a white to pale yellow lyophilized powder from a buffer containing 10 mM CH3COONH4. No preservatives are added. The purity of WGA is determined by SDS- electrophoresis which generates three bands, corresponding to the three isolectins of WGA. Protein content is verified in spectrophotmetry The lectin is available in vials containing 100 mg, 25 mg or 10 mg powder and the product is to be used for laboratory work only.
應用
使用指南
凝膠素可以在使用前用2ml去離子水重構。輕輕旋轉小瓶,直至*溶解。認為在高濃度的2-巰基乙醇的存在下發生聚集。
運輸和存儲
該產品的運輸時間為-20°C,但對于日常運輸,可能會在環境溫度下運輸。冷凍干燥粉從生產之日起至少保持在-20℃以下穩定三年以上。用去離子水重構后,溶液可以冷凍保存在等分試樣中長達12個月。
參考
(1) Johnson R. J., Simpson S., Van Epps D. E., Chenoweth D. E. (1992) Wheat germ agglutinin inhibits the C receptor interaction: implications for receptor microheterogeneity and ligand binding site. Journal of Leukocyte Biology Vol 52, Issue 1, 3–10.
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