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上海起福生物科技有限公司Santa Cruz專業代理,具體產品信息歡迎電詢:
Santa Cruz Biotechnology
TECHNICAL SERVICE GUIDE: Western Blotting
Catalog #: Lot #:
PROBLEM AND PREVIOUS EXPERIENCE
- What is the specific problem you are experiencing?
- What size bands were expected and what size bands were detected?
- Was the blot blank or was a dark background or non-specific bands seen?
- Did this same vial of product work in the past? What were the results?
- Did other lots of this product work in the past? Which lots? What were the results?
SAMPLES AND CONTROLS
- From what species (animal) was the sample and what type of sample was used (cell lysate, tissue, purified protein, etc.)? What lysis buffer was used?
- How much sample was loaded on the gel?
- If the blocking peptide was used, was it used as a negative control?
- What positive and/or negative controls were used, and what were the results?
BLOCKING
- What were the blocking conditions (blocking solution, how long, what temperature. Etc.)?
上海起福生物科技有限公司
:楊建輝
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辦公:
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