型號 | 載體名稱 | 出品公司 | 載體用途 |
VPI0021 | pCEP4(附加體形式) | Invitrogen | 哺乳動物表達載體 |
Key Function:Episomal Expression
Mammalian Selection:Hygromycin
Vector Type:pCEP4
Cloning Method:Restriction Enzyme/MCS
Protein Tag or Fusion:Untagged
Constitutive or Inducible System:Constitutive
pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells.
pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.
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