型號 | 載體名稱 | 出品公司 | 載體用途 |
VMA0372 | pSicoR | Addgene | 慢病毒載體 |
Backbone size (bp): 7567
Modifications to Backbone: EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5': HpaI
Cloning site 3': XhoI
5' sequencing primer: mU6-F
pSicoR allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression.
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