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世界*實(shí)驗(yàn)材料供應(yīng)商 Cell Technology正式授權(quán)上海起發(fā)為其中國(guó)代理， Cell Technology在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國(guó)科研客戶帶來(lái)的產(chǎn)品，的服務(wù)，簽約 Cell Technology就是為了給廣大科研客戶帶來(lái)更加完善的產(chǎn)品和服務(wù),您的滿意將是我們zui大的收獲
Cell Technology中國(guó)代理, Cell Technology上海代理， Cell Technology北京代理，Cell Technology廣東代理， Cell Technology江蘇代理Cell Technology湖北代理,Cell Technology天津,Cell Technology黑龍江代理,Cell Technology內(nèi)蒙古代理,Cell Technology吉林代理,Cell Technology福建代理, Cell Technology江蘇代理, Cell Technology浙江代理, Cell Technology四川代理,
Cell Technology成立于1998年，致力于為研究院、生物技術(shù)公司及制藥企業(yè)的科研人員研發(fā)生產(chǎn)*的細(xì)胞功能研究用試劑與試劑盒。這些檢測(cè)試劑與試劑盒可以實(shí)現(xiàn)不破壞細(xì)胞形態(tài)和結(jié)構(gòu)的情況下原位檢測(cè)細(xì)胞成分，研究細(xì)胞內(nèi)發(fā)生的特定事件.Cell Technology發(fā)布了通過(guò)測(cè)定胞內(nèi)活性caspase及線粒體膜電位研究細(xì)胞凋亡的一系列試劑。隨著公司研發(fā)團(tuán)隊(duì)的持續(xù)努力，我們又推出了一系列高特異性的caspase檢測(cè)試劑，以及cathepsin檢測(cè)的試劑和ELISA試劑盒。目前Cell Technology提供的檢測(cè)試劑和ELISA試劑盒涵蓋Caspase、NOS、ROS、線粒體膜電位、信號(hào)通路和蛋白磷酸化等多個(gè)研究領(lǐng)域，產(chǎn)品倍各大高校、研究機(jī)構(gòu)、生物技術(shù)企業(yè)和制藥企業(yè)采用。
Cell Technology主要產(chǎn)品簡(jiǎn)介
1、Fluoro Cholesterol：高靈敏、一步檢測(cè)生物樣本中總膽固醇含量，可用于細(xì)胞代謝與藥物作用的研究。
2、Fluoro NADP：檢測(cè)組織或細(xì)胞提取液內(nèi)NADP/NADPH含量，與NAD/NADH檢測(cè)無(wú)交叉，可用于細(xì)胞死亡、抗氧化/氧化應(yīng)激、能量代謝及線粒體功能研究。
3、Fluoro Sarcosine：定量檢測(cè)細(xì)胞、血清及組織提取物中的肌氨酸含量。
4、Fluoro IndoBlu：定量檢測(cè)細(xì)胞中IndoBlu的含量，適用于細(xì)胞增殖研究。
5、Fluoro Lactate：定量檢測(cè)生物樣本中 L-lactate的含量。
6、Fluoro Phosphate：定量檢測(cè)生物樣本中的磷含量，靈敏度可達(dá)200nM。
Fluoro NADP
Fluorescent NADP/NADPH Detection Kit - Detection of NADP/NADPH in tissue extracts /cell samples
| Fluoro NADP™ NEW! Fluorescent NADP/NADPH Detection Kit - Detection of NADP/NADPH in tissue extracts /cell samples | |||||||||
| Key Benefits: | |||||||||
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| Introduction | |||||||||
Nicotinamide adenine dinucleotide phosphate (NADP+) is used in anabolic reactions, such as lipid and nucleic acid synthesis, which require NADPH as a reducing agent. NADPH is the reduced form of NADP+, and NADP+ is the oxidized form of NADPH. In cells, NADPH plays the role of a carrier of reducing power and is primarily involved in maintaining optimal redox metabolism. A simplified assay for the measurement of NAD and NADP is critical to understanding the roles of these pyridine nucleotides in normal and abnormal cells. | |||||||||
| Assay Principle | |||||||||
| The Fluoro NADP/NADPH detection kit utilizes a non-fluorescent detection reagent, which is oxidized in the presence NADPH to produce its fluorescent analog and NADP. NADP is further converted to NADPH via an enzyme-coupled reaction. The enzyme reaction specifically reacts with NADP/NADPH and not with NAD/NADH. Reaction: 1. NADPH + non-fluorescent detection reagent + electron coupler → fluorescent analog + NADP 2. NADP + enzyme coupled reaction → NADPH (then proceeds to reaction 1). Excitation: 530-570nm and Emission at 590-600nm | |||||||||
| References | |||||||||
| 1. Comprehensive Invited Review WEIHAI YING. NAD /NADH and NADP /NADPH in Cellular Functions and Cell Death: Regulation and Biological Consequences. ANTIOXIDANTS &REDOX SIGNALING Volume 10,Number 2,2008. 2. Bedard K and Krause KH. The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology.Physiol Rev 87:245 –313,2007. 3. Lowry, Oliver H., Passonneau, Janet V. and Rock, Martha K. The Stability of Pyridine Nucleotides. The Journal of Biological Chemistry, 236, #10, 1961. | |||||||||
| Kit contents (for 100 assays) | |||||||||
| 1. Enzyme Mix 170μL Part # 6022 .......................... 4-8C 2. NADP/NADPH Lysis Solution 12.5mL Part# 3050 ............................4-8C 3. NADP Extraction Buffer 22 mL Part# 3046 (Green label) ..........4-8C 4. NADPH Extraction Buffer 22 mL Part# 3047 (Magenta label)........4-8C 5. Reaction Buffer 11 mL. Part# 3049 ............................4-8C 6. Standard curve diluent 15 ml. Part # 3052............................4-8C 7. NADPH Detection reagent 110 μL Part # 4019...........................-20C 8. 3X Substrate Mix 6mL Part # 3048........................... -20C 9. NADPH Standard- 3 vials dried Part# 7014.............................-20C | |||||||||
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