世界*實驗材料供應商 Viagen Biotech, Inc.正式授權上海起發為其中國代理, Viagen Biotech, Inc.在一直是行業的*,一直為廣大科研客戶提供zui為的產品和服務,上海起發一直秉承為中國科研客戶帶來的產品,的服務,簽約 Viagen Biotech, Inc.就是為了給廣大科研客戶帶來更加完善的產品和服務,您的滿意將是我們zui大的收獲
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Viagen Biotech Inc.是創新的DNA提取技術和生命科學研究產品的供應商。ViagenDirectPCR®DNA提取系統是一種單管系統,用于從小鼠尾巴,耳朵片,卵黃囊和培養細胞中快速制備DNA。由Viagen Biotech Inc.的科學家開發的正在申請的組件允許得到的DNA提取物與用于基因分型的基因組PCR相容。生物樣品的粗提取物與許多分子生物學級反應(如聚合酶鏈反應(PCR))不相容,部分原因是粗提物中含有抑制劑。DirectPCR試劑不僅介導生物樣品的快速溶解,而且還含有有效抑制粗裂解物對PCR擴增的抑制活性的抑制劑,同時zui大限度地保持了釋放的基因組DNA的完整性。該過程*消除了任何溶液轉移或開管步驟,節省了大量的時間和精力。DirectPCR試劑每尾只花費26美分,可以節省任何使用量。
Viagenbiotech致力于簡便快速提取和制備核酸(DNA / RNA),以批量、快速進行基因分析。公司擁有的技術不僅加快樣品的裂解,而且降低了粗提物中PCR抑制因素,因而使DNA提取與PCR基因分析兼容,樣品經一步消化后即可進行PCR擴增,并且保證了基因組的完整性。
Availability: In stock
Genomic PCR Grade Proteinase K Solution
Catalog No. 501-PK
Stable for 12 months at -20℃ / Ships Refrigerated Fedex Overnight
Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.
DNA Extraction lysis reagent for DNA tests & genotyping: The Proteinase K solution is useful when a small amount of biological samples are processed. Add the enzyme solution to DirectPCR reagents at the final concentration of 0.5-1.0 mg/ml (25-50 μl Proteinase K solution per 1 ml DirectPCR reagent). Follow the instruction manuals for DirectPCR Reagent Protocol/Manual for each blood, blood stain, buccal swab, hair root DNA genotyping. Once diluted in DirectPCR reagents, Proteinase K is stable for ~24 hrs.
Other applications: Proteinase K digests native proteins very effectively. It can therefore be used to remove DNases and RNases when isolating DNA and RNA from tissues or cells.
Usage Conditions:
• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At high temperatures—optimum digestion temperature is 65°C
Unit Definition: One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.
Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases
部分產品報價如下:
貨號 | 品名 | 規格 | 價格 | 品牌 |
101-T | DirectPCR Lysis Reagent (mouse tail) | 50mL | 1160 | Viagen Biotech, Inc. |
102-T | DirectPCR Lysis Reagent (mouse tail) | 100mL | 2040 | Viagen Biotech, Inc. |
201-Y | DirectPCR Lysis Reagent (yolk sac) | 50mL | 1160 | Viagen Biotech, Inc. |
202-Y | DirectPCR Lysis Reagent (yolk sac) | 100mL | 2040 | Viagen Biotech, Inc. |
301-C | DirectPCR Lysis Reagent (cell) | 50mL | 1160 | Viagen Biotech, Inc. |
302-C | DirectPCR Lysis Reagent (cell) | 100mL | 2040 | Viagen Biotech, Inc. |
401-E | DirectPCR Lysis Reagent (mouse ear) | 25mL | 1160 | Viagen Biotech, Inc. |
402-E | DirectPCR Lysis Reagent (mouse ear) | 50mL | 2040 | Viagen Biotech, Inc. |
21141 | Redsafe Nucleic Acid Staining Solution | 1ml | 1180 | Viagen Biotech, Inc. |
505-PKP | Genomic PCR Grade Proteinase K Powder | 100mg | 1300 | Viagen Biotech, Inc. |
506-PKP | Genomic PCR Grade Proteinase K Powder | 200mg | 2200 | Viagen Biotech, Inc. |
507-PKP | Genomic PCR Grade Proteinase K Powder | 500mg | 4700 | Viagen Biotech, Inc. |
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1:基本什么都能進口,血清,抗體,耗材,還有部分限制進口的,
2:貨品全,現經營過700多個品牌,基本所有生物試劑耗材都可以進口,特別是冷偏的產品那就更有優勢,
3:提供加急服務,一般1-2周到貨,超過時限加急費全免
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5:良好的信譽,大部分客戶我們提供貨到付款服務,客戶包括清華,北大 交大 復旦,中山等100多所大學,ROCHE,阿斯利康,國藥 ,fisher等500多家公司
6:我們*代理的品牌有:Antibody Research Corporation,arcticzymes,Biorelevant,AmberGen, Inc. ,clemente-associates,clodronaiposomes,Columbia Biosciences,enzyme research,Gene Bridges GmbH,Genovis,AmberGen, Inc. Biotechnology GmbH,Haematologic Technologies HTI Haemtech,hookelabs,Immudex,Innovative Research of America,inspiralis ,List Biological Laboratories, Inc.,lumafluor,Microsurfaces,multiplicom,nanotools,Pel-Freez Biologicals,pentapharm,progen,Protein Ark,QA-Bio,Inc,QA-Bio,IncQuickZyme Biosciences,Teknova,TriLink BioTechnologies, Inc.,Zyagen Laboratories 等
7:我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發,歡迎合作。
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